Jordi NP DVB Polyamino

NP DVB Polyamino columns contain secondary amino groups which quaternize in acidic solutions allowing mixed mode ion exchange and normal phase separations. Normal phase separations are used to separate molecules based on their polarity as opposed to their hydrophobiicty as in RP. Weakly polar compounds are found to elute first followed by more polar components. This can be used to fractionate a sample based on the functional groups present in each molecule. Gradients are the opposite of those typically used for RP chromatography and thus a gradient from a non-polar solvent to a more polar solvent is used.

Jordi NP DVB Polyamino columns provide the following benefits:

· class based separations
· high column plate counts
· mixed mode separations combining ion exchange and normal phase
· rapid recovery of the phase following gradient analysis
· rugged - solvent changes will not damage columns
· solvent compatibility - 100% organic to 100% aqueous buffers
· wide pH range - pH values from 0 to 14.
· long column life - 3 month warranty standard

 

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