RP Best Results
Tips for Best Results with Reverse Phase Columns
1. Run your reverse phase columns at the following flow rates for best results.
| Column ID (mm) | Flow Rate (ml/min) |
| 4.6 | 0.5-1.5 |
| 10 | 1.0-2.0 |
| 22 | 3.0-6.0 |
2. Do not worry about high back pressures. Jordi columns are packed at 8,000psig and can run for months at pressures in the 3,000-5,000psig range without harming them.
3. If you notice a calibration change after significant use, you may need a clean frit(s), particularly on the column inlet.
4. Try to keep at least 10% organic in your solvent if possible. Because the gel is very hydrophobic and will not wet in water, we cannot pack your column in pure water. When pure water/buffer is used as mobile phase, the gel will shrink slightly and can cause a premature loss in column efficiency.